Sequence: KFFKFFKFFK
PNA (antisense; scrambled control)
| Experiment Id | EXP001060 |
|---|---|
| Paper | Identifying Antisense Oligonucleotides to Disrupt Small RNA Regulated Antibiotic Resistance via a Ce |
| Peptide | (KFF)3K |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | 6–8 µM final (used as control in reporter/MIC assays) |
| Rna Concentration | 6 µM (control vs 12-1); 6 µM in combo-control; 8 µM tested as higher-dose control |
| Mixing Ratio | N/A (covalent conjugate) |
| Formulation Format | CPP-PNA conjugate (covalent); added directly to E. coli cultures / MIC wells |
| Formulation Components | KFFKFFKFFK-O-ttccgccaactc (SI Table S3) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Escherichia coli MG1655 (liquid culture reporter assay and MIC assays) |
| Animal Model | |
| Administration Route | |
| Output Type | reporter fluorescence control; MIC control; growth effect at higher dose |
| Output Value | 6 µM scramble used as control; 8 µM scramble decreased growth rate and also reduced MIC (nonspecific effect) |
| Output Units | |
| Output Notes | Control CPP-PNA scramble used throughout; authors note 8 µM scramble reduced growth rate and reduced MIC, suggesting concentration-dependent nonspecific effects. |
| Toxicity Notes | 8 µM CPP-PNA scramble decreased growth rate; reduction in MIC at 8 µM likely nonspecific. |
| Curation Notes |