Sequence: KFFKFFKFFK
| Experiment Id | EXP001061 |
|---|---|
| Paper | Identifying Antisense Oligonucleotides to Disrupt Small RNA Regulated Antibiotic Resistance via a Ce |
| Peptide | (KFF)3K |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 6 µM final (reporter & MIC assays); 3 µM tested (no effect reported); also used 3 µM in combo |
| Rna Concentration | 6 µM final (alone); 3 µM final (in combination with 33-22) |
| Mixing Ratio | N/A (covalent conjugate) |
| Formulation Format | CPP-PNA conjugate (covalent); added directly to E. coli cultures / MIC wells |
| Formulation Components | KFFKFFKFFK-O-aatgatgatagc (SI Table S3) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Escherichia coli MG1655 (liquid culture reporter assay and MIC assays) |
| Animal Model | |
| Administration Route | |
| Output Type | ompF-sfgfp reporter derepression; MIC (cephalothin) test |
| Output Value | At 6 µM, reporter fluorescence increased to near reporter-only control after 1 h; MIC for cephalothin showed no difference vs scramble (variability noted) |
| Output Units | |
| Output Notes | Authors report 6 µM CPP-PNA 12-1 restored reporter signal but MIC improvement was not observed for cephalothin; also note unexplained increased fluorescence in some controls with 6 µM 12-1. |
| Toxicity Notes | |
| Curation Notes |