Sequence: KFFKFFKFFK
PNA (antisense to MicF; 33-22)
| Experiment Id | EXP001062 |
|---|---|
| Paper | Identifying Antisense Oligonucleotides to Disrupt Small RNA Regulated Antibiotic Resistance via a Ce |
| Peptide | (KFF)3K |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | 3–4 µM final (tested in combination with 12-1) |
| Rna Concentration | 3–4 µM final (tested in combination with 12-1) |
| Mixing Ratio | N/A (covalent conjugate) |
| Formulation Format | CPP-PNA conjugate (covalent); used mainly in combination |
| Formulation Components | KFFKFFKFFK-O-atgacggtaata (SI Table S3) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Escherichia coli MG1655 (liquid culture reporter assay and MIC assays) |
| Animal Model | |
| Administration Route | |
| Output Type | TX-TL / reporter screening; combination MIC |
| Output Value | Little/no MicF–ompF inhibition when used alone in TX-TL; enabled activity when combined with 12-1 |
| Output Units | |
| Output Notes | Paper notes anti-MicF 33-22 alone did not inhibit MicF–ompF binding in TX–TL, but improved inhibition when combined with 12-1. |
| Toxicity Notes | |
| Curation Notes |