Sequence: rRrGrKkRr
DNA oligonucleotide probe (ECHO)
| Experiment Id | EXP001074 |
|---|---|
| Paper | Hybridization-Sensitive Fluorescent Oligonucleotide Probe Conjugated with Cell-Penetrating Peptides |
| Peptide | cTAT |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | no |
| Peptide Concentration | 50 µM (probe/conjugate) |
| Rna Concentration | 50 µM |
| Mixing Ratio | |
| Formulation Format | lipoplex (Lipofectamine LTX-assisted) |
| Formulation Components | CPP–ECHO conjugate + Lipofectamine LTX; CuAAC triazole linker |
| Size Nm | 100.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa |
| Animal Model | |
| Administration Route | |
| Output Type | cell penetration rate (% fluorescent cells; live-cell fluorescence microscopy) |
| Output Value | 64.4% fluorescent cells |
| Output Units | |
| Output Notes | With Lipofectamine LTX: 1 h incubation; mean percent fluorescent cells counted (Fig.4B). DLS showed sub-visible aggregates (radius ~100 nm) in reaction mixture; dissociated by NaCl before purification. |
| Toxicity Notes | Cytotoxicity of conjugate or conjugate-included lipoplex negligible in 1 h treatment |
| Curation Notes |