RNA toehold switch-based reporter assay to assess bacterial uptake of antisense oligomers (2025)
Sequence: GRRRRRRRRRPPQ
| Experiment Id | EXP001079 |
|---|---|
| Paper | RNA toehold switch-based reporter assay to assess bacterial uptake of antisense oligomers |
| Peptide | R9-TAT |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 5 µM (CPP-PNA) |
| Rna Concentration | 5 µM (PNA as CPP-PNA) |
| Mixing Ratio | 1:1 (covalent CPP-PNA) |
| Formulation Format | CPP–PNA conjugate (no linker) |
| Formulation Components | CPP and PNA synthesized on same resin; direct conjugate (no linker); used as antisense activator in bacteria |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Salmonella enterica serovar Typhimurium SL1344 carrying TS7-Mut_9::sfGFP reporter plasmid; MHB medium |
| Animal Model | |
| Administration Route | |
| Output Type | Reporter activation (sfGFP fold upregulation) |
| Output Value | |
| Output Units | |
| Output Notes | CPP conjugated to PNA_toe (11-mer) used to activate TS7-Mut_9::sfGFP toehold switch reporter; fluorescence measured by plate reader / flow cytometry as proxy for cytosolic PNA delivery. TAT, R9-TAT, and L5a showed negligible activation in Salmonella. |
| Toxicity Notes | |
| Curation Notes |