Sequence: KMTRAQRRAAARRNRWTAR
| Experiment Id | EXP001094 |
|---|---|
| Paper | To conjugate or not to conjugate? evaluating the potential use of cell-penetrating peptides for conj |
| Peptide | BMV Gag |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | 100 nM and 400 nM (SPR injections) |
| Rna Concentration | 1 µM biotinylated siRNA immobilized on SA chip (200 s) |
| Mixing Ratio | N/A (SPR binding screen; immobilized siRNA) |
| Formulation Format | Noncovalent CPP–siRNA binding screen by SPR (pH 7.4) |
| Formulation Components | BIACORE T200; SA sensor chip with biotin-siRNA; HBS-EP+ running buffer |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | |
| Animal Model | |
| Administration Route | |
| Output Type | SPR binding stoichiometry (CPP per siRNA) |
| Output Value | 4.8 |
| Output Units | |
| Output Notes | Charge-to-length ratio: 0.4; charge at pH 7.5: 8.0; theoretical MW (Da): 2426.0. |
| Toxicity Notes | |
| Curation Notes |