Sequence: GLWRALWRLLRSLWRLLWKA
siRNA (multimeric antisense/guide strands annealed to PNA sense)
| Experiment Id | EXP001147 |
|---|---|
| Paper | HER2 RNA Aptamer- and Cell Penetrating Peptide-Mediated Delivery of Multimeric Antisense Strands of |
| Peptide | CADY |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 500 nM (reported as monomeric antisense equivalents) |
| Rna Concentration | 500 nM (monomeric antisense equivalents) |
| Mixing Ratio | Equimolar RNA:PNA in annealing buffer (Tris/NaCl); CPP is covalently linked to PNA |
| Formulation Format | CPP–PNA-annealed multimeric siRNA (hybridized multimeric antisense + CPP-PNA sense) |
| Formulation Components | Multimeric antisense (HS-UGCGCUCCUGGACGUAGCCUU-SH) polymer + CADY-8mer PNA (Ac-GGAGCGCA-GLWRALWRLLRSLWRLLWKA) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | SKOV-3 (HER2-positive, eGFP-expressing ovarian carcinoma cells) |
| Animal Model | |
| Administration Route | |
| Output Type | Functional gene silencing (eGFP fluorescence decrease; microscopy quantification) |
| Output Value | Significant eGFP knockdown vs no siRNA (quantified fluorescence intensity; 24 h images and time-course) |
| Output Units | |
| Output Notes | CPP-PNA-annealed multimeric siRNA showed substantial gene silencing in SKOV-3 without lipofectamine; slower kinetics vs lipofectamine control (time-course). |
| Toxicity Notes | |
| Curation Notes |