Sequence: ETWW
| Experiment Id | EXP001150 |
|---|---|
| Paper | Advancing Antisense Oligonucleotide Delivery through Click Chemistry Based Chemical Conjugation with |
| Peptide | ETWW |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | medium |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | covalent CPP–ASO conjugate (CuAAC click chemistry) |
| Formulation Components | ETWW peptide + exon51 ASO via triazole linker |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | C2C12 (MTT cytotoxicity assay on CPP/CPP-ASO constructs) |
| Animal Model | |
| Administration Route | |
| Output Type | cytotoxicity |
| Output Value | MTT viability reported (see Supplementary Figure SF56) |
| Output Units | |
| Output Notes | CPP and CPP–ASO conjugates synthesized via click chemistry; biological characterization beyond cytotoxicity not clearly described for this variant in the main text. |
| Toxicity Notes | C2C12 MTT assay performed on CPPs and CPP–ASO conjugates across dose range; arginine-containing variants noted as more cytotoxic. |
| Curation Notes |