Sequence: HHHCRRRRRC
| Experiment Id | EXP001159 |
|---|---|
| Paper | A biodegradable stearylated peptide with internal disulfide bonds for efficient delivery of siRNA in |
| Peptide | HRss2 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | N/P=10 formulation; polymer amount depends on N/P (1.06 µg polymer per 1 µg siRNA corresponds to N/P=1) |
| Rna Concentration | 70 nM siLuc (gene silencing vs N/P, 24 h); also tested 100 nM for decay kinetics |
| Mixing Ratio | N/P ratio (amine/phosphate); optimum reported N/P=10 |
| Formulation Format | Electrostatic polyplex (SHR/SHRss variants with siRNA) prepared in PBS pH 6.0, incubated 30 min |
| Formulation Components | SHRss (or control polymer) + siLuc in PBS (pH 6.0) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Luc-Hela cells |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro functional gene silencing (luciferase activity reduction) |
| Output Value | Significant luciferase knockdown; SHRss2 reported highest silencing at N/P=10 vs other SHRss and SHR |
| Output Units | |
| Output Notes | Reporter assay at 24 h post-transfection; luminescence normalized to protein; SHRss2 also showed slower knockdown decay over 5 days after single 100 nM siLuc treatment. |
| Toxicity Notes | Low cytotoxicity reported for SHRss polyplexes (>90% viability up to 200 nM siRNA under transfection conditions). |
| Curation Notes |