Sequence: Stearyl-HHHCRRRRRC
| Experiment Id | EXP001161 |
|---|---|
| Paper | A biodegradable stearylated peptide with internal disulfide bonds for efficient delivery of siRNA in |
| Peptide | SHRss2 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | N/P=10 formulation (optimum used for transfection) |
| Rna Concentration | 100 nM simCh |
| Mixing Ratio | N/P=10 |
| Formulation Format | Electrostatic polyplex (SHRss2/simCh) prepared in PBS pH 6.0, incubated 30 min |
| Formulation Components | SHRss2 + simCh in PBS (pH 6.0) |
| Size Nm | 163.00 |
| Zeta Mv | 24.60 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | mCherry-HEK293 cells |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro functional gene silencing (mCherry/RFP signal reduction) |
| Output Value | Weaker red fluorescence and lower RFP-positive cells vs controls |
| Output Units | |
| Output Notes | Assessed by confocal imaging and flow cytometry at 24 h post-transfection. |
| Toxicity Notes | High viability (>90%) under transfection conditions up to 200 nM siRNA. |
| Curation Notes |