Sequence: Stearyl-HHHCRRRRRC
| Experiment Id | EXP001164 |
|---|---|
| Paper | A biodegradable stearylated peptide with internal disulfide bonds for efficient delivery of siRNA in |
| Peptide | SHRss2 |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | Formulated at N/P=10 |
| Rna Concentration | 1.5 mg/kg siRNA daily for 3 days |
| Mixing Ratio | N/P=10 |
| Formulation Format | Electrostatic polyplex (SHRss2/siLuc) |
| Formulation Components | SHRss2 + siLuc in PBS (pH 6.0) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Female Balb/c nude mice bearing Luc-Hela xenograft tumors (subcutaneous) |
| Administration Route | Intravenous (tail vein); imaging uses intraperitoneal D-luciferin |
| Output Type | In vivo functional gene silencing (tumor bioluminescence + luciferase assay) |
| Output Value | Reduced tumor luminescence and decreased luciferase activity vs SHRss2/NC-siRNA control |
| Output Units | |
| Output Notes | IVIS imaging before/after dosing; tumor lysate luciferase assay shows significant reduction vs control. |
| Toxicity Notes | |
| Curation Notes |