Sequence: CKRRMKWKKC
| Experiment Id | EXP001165 |
|---|---|
| Paper | Macrophage membrane- and cRGD-functionalized thermosensitive liposomes combined with CPP to realize |
| Peptide | CPP |
| Delivery Success Class | no |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | CPP covalently attached to siRNA (C-siRNA) |
| Rna Concentration | 100 nM (in vitro assays) |
| Mixing Ratio | 1:1 (covalent conjugate) |
| Formulation Format | Free CPP–siRNA conjugate (C-siRNA; disulfide-linked CPP to siRNA) |
| Formulation Components | CPP (CKRRMKWKK-Cys) disulfide-linked to thiol-modified Bcl-2 siRNA (C-siRNA) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HepG2 human hepatocellular carcinoma cells |
| Animal Model | |
| Administration Route | |
| Output Type | Bcl-2 mRNA and protein suppression (qPCR, western blot); apoptosis |
| Output Value | Strongest suppression among unheated groups (comparable to heated MTSLR) |
| Output Units | |
| Output Notes | Free C-siRNA showed high cellular uptake and strong Bcl-2 suppression; also induced high apoptosis. |
| Toxicity Notes | C-siRNA caused strongest proliferation inhibition vs other groups in HepG2 (time-dependent). |
| Curation Notes |