Sequence: CKRRMKWKKC
| Experiment Id | EXP001166 |
|---|---|
| Paper | Macrophage membrane- and cRGD-functionalized thermosensitive liposomes combined with CPP to realize |
| Peptide | CPP |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Encapsulated C-siRNA (CPP-siRNA conjugate) |
| Rna Concentration | 100 nM (in vitro assays) |
| Mixing Ratio | Encapsulated (EE ~89%) |
| Formulation Format | C-siRNA encapsulated thermosensitive liposome (C-siRNA/TSL) |
| Formulation Components | Thermosensitive liposomes (DPPC:MSPC:DSPE-PEG2000 165:5.5:5 mol%); macrophage membrane coating (from RAW264.7) and DSPE-PEG2000-cRGD insertion for MTSLR; C-siRNA encapsulated; hyperthermia 42°C triggers release. |
| Size Nm | 86.90 |
| Zeta Mv | -8.20 |
| Model Scope | in_vivo |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HepG2 human hepatocellular carcinoma cells |
| Animal Model | |
| Administration Route | |
| Output Type | Bcl-2 mRNA and protein suppression (qPCR, western blot); apoptosis |
| Output Value | Moderate suppression (less than MTSL/MTSLR; enhanced vs siRNA/TSL and free siRNA) |
| Output Units | |
| Output Notes | In vitro uptake and gene silencing observed; efficacy ranked below MTSL and MTSLR. |
| Toxicity Notes | Reduced viability vs control; less than free C-siRNA. |
| Curation Notes |