Sequence: CKRRMKWKKC
| Experiment Id | EXP001168 |
|---|---|
| Paper | Macrophage membrane- and cRGD-functionalized thermosensitive liposomes combined with CPP to realize |
| Peptide | CPP |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Encapsulated C-siRNA |
| Rna Concentration | 100 nM (in vitro assays); uptake assay 100 nM cy5-siRNA |
| Mixing Ratio | Encapsulated (EE ~86%) |
| Formulation Format | Macrophage membrane- and cRGD-functionalized C-siRNA thermosensitive liposome (C-siRNA/MTSLR) |
| Formulation Components | Thermosensitive liposomes (DPPC:MSPC:DSPE-PEG2000 165:5.5:5 mol%); macrophage membrane coating (from RAW264.7) and DSPE-PEG2000-cRGD insertion for MTSLR; C-siRNA encapsulated; hyperthermia 42°C triggers release. |
| Size Nm | 108.20 |
| Zeta Mv | -15.00 |
| Model Scope | in_vivo |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HepG2 human hepatocellular carcinoma cells |
| Animal Model | |
| Administration Route | |
| Output Type | Bcl-2 mRNA and protein suppression (qPCR, western blot); apoptosis |
| Output Value | High suppression (below heated MTSLR; above MTSL) |
| Output Units | |
| Output Notes | cRGD + macrophage membrane enhanced uptake and gene silencing in HepG2 vs other nanoparticles (without heat). |
| Toxicity Notes | |
| Curation Notes |