Sequence: CKRRMKWKKC
| Experiment Id | EXP001169 |
|---|---|
| Paper | Macrophage membrane- and cRGD-functionalized thermosensitive liposomes combined with CPP to realize |
| Peptide | CPP |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Encapsulated C-siRNA; heated to trigger release |
| Rna Concentration | 100 nM (in vitro assays) |
| Mixing Ratio | Encapsulated; heat-triggered release (~80% in 10 min at 42°C) |
| Formulation Format | C-siRNA/MTSLR with hyperthermia (42°C; heated) (C-siRNA/MTSLR-H) |
| Formulation Components | Thermosensitive liposomes (DPPC:MSPC:DSPE-PEG2000 165:5.5:5 mol%); macrophage membrane coating (from RAW264.7) and DSPE-PEG2000-cRGD insertion for MTSLR; C-siRNA encapsulated; hyperthermia 42°C triggers release. |
| Size Nm | 108.20 |
| Zeta Mv | -15.00 |
| Model Scope | in_vivo |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HepG2 human hepatocellular carcinoma cells |
| Animal Model | |
| Administration Route | |
| Output Type | Bcl-2 mRNA and protein suppression (qPCR, western blot); apoptosis |
| Output Value | Strongest suppression among nanoparticle groups |
| Output Units | |
| Output Notes | With heat-triggered release, C-siRNA/MTSLR-H showed highest uptake, apoptosis, and Bcl-2 knockdown among formulations. |
| Toxicity Notes | |
| Curation Notes |