Sequence: CKRRMKWKKC
| Experiment Id | EXP001173 |
|---|---|
| Paper | Macrophage membrane- and cRGD-functionalized thermosensitive liposomes combined with CPP to realize |
| Peptide | CPP |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | medium |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | Encapsulated C-siRNA |
| Rna Concentration | 1.2 mg/kg (siRNA equivalent) |
| Mixing Ratio | Encapsulated; heat-triggered release |
| Formulation Format | C-siRNA/MTSL with hyperthermia (42°C) |
| Formulation Components | Thermosensitive liposomes (DPPC:MSPC:DSPE-PEG2000 165:5.5:5 mol%); macrophage membrane coating (from RAW264.7) and DSPE-PEG2000-cRGD insertion for MTSLR; C-siRNA encapsulated; hyperthermia 42°C triggers release. |
| Size Nm | 105.30 |
| Zeta Mv | -18.90 |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Male nude mice bearing subcutaneous HepG2 tumor xenografts |
| Administration Route | Intravenous (tail vein); tumor region heated to 42°C for 30 min after injection (except U group). |
| Output Type | Tumor growth inhibition + Bcl-2 mRNA/protein suppression in tumors |
| Output Value | Strong tumor inhibition (below MTSLR) |
| Output Units | |
| Output Notes | Macrophage membrane coating improved tumor accumulation and efficacy vs TSL; heated after injection. |
| Toxicity Notes | |
| Curation Notes |