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EXP001176

Paper

Macrophage membrane- and cRGD-functionalized thermosensitive liposomes combined with CPP to realize precise siRNA delivery into tumor cells (2022)

Peptide

CPP

Sequence: CKRRMKWKKC

RNA

siRNA

All experiment fields

Experiment Id EXP001176
Paper Macrophage membrane- and cRGD-functionalized thermosensitive liposomes combined with CPP to realize
Peptide CPP
Delivery Success Class no
In Vivo Flag yes
Uptake Confirmed no
Label Confidence medium
In Vitro Functional Effect
Endosomal Escape Evidence
Peptide Concentration Encapsulated C-si.NC
Rna Concentration 1.2 mg/kg (siRNA equivalent)
Mixing Ratio Encapsulated; heat-triggered release
Formulation Format C-siN.C/MTSLR with hyperthermia (42°C) (negative-control siRNA)
Formulation Components Thermosensitive liposomes (DPPC:MSPC:DSPE-PEG2000 165:5.5:5 mol%); macrophage membrane coating (from RAW264.7) and DSPE-PEG2000-cRGD insertion for MTSLR; C-siRNA encapsulated; hyperthermia 42°C triggers release.
Size Nm 108.20
Zeta Mv -15.00
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model Male nude mice bearing subcutaneous HepG2 tumor xenografts
Administration Route Intravenous (tail vein); tumor region heated to 42°C for 30 min after injection (except U group).
Output Type Tumor growth inhibition / gene silencing controls
Output Value No therapeutic tumor inhibition; used as negative control
Output Units
Output Notes Negative-control CPP-siRNA in MTSLR with heat did not produce therapeutic effects compared with Bcl-2 siRNA formulations.
Toxicity Notes
Curation Notes