Sequence: CKRRMKWKK
| Experiment Id | EXP001177 |
|---|---|
| Paper | Co-delivery of CPP decorated doxorubicin and CPP decorated siRNA by NGR-modified nanobubbles for imp |
| Peptide | CPP |
| Delivery Success Class | no |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | CPP covalently linked to siRNA (CPP–siRNA) |
| Rna Concentration | 100 nM siRNA; 4 h treatment then 48 h before qRT-PCR |
| Mixing Ratio | Equimolar CPP and siRNA during conjugation (reported 6 µM synthesis conditions) |
| Formulation Format | Free CPP–siRNA conjugate (no carrier) |
| Formulation Components | CPP–siRNA conjugate prepared via disulfide linkage |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HT-1080 human fibrosarcoma cells |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro functional RNA effect (qRT-PCR c-myc mRNA suppression) |
| Output Value | Improved c-myc suppression vs free siRNA |
| Output Units | |
| Output Notes | CPP conjugation increased gene silencing compared with free siRNA. |
| Toxicity Notes | |
| Curation Notes |