Sequence: CKRRMKWKK
| Experiment Id | EXP001179 |
|---|---|
| Paper | Co-delivery of CPP decorated doxorubicin and CPP decorated siRNA by NGR-modified nanobubbles for imp |
| Peptide | CPP |
| Delivery Success Class | no |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Encapsulated CPP–siRNA |
| Rna Concentration | 100 nM siRNA; 4 h treatment then 48 h before qRT-PCR |
| Mixing Ratio | Encapsulated (dialysis to remove unencapsulated cargo) |
| Formulation Format | CPP–siRNA loaded nanobubbles (NB) with ultrasound pre-treatment (+US) |
| Formulation Components | NBs prepared from HSPC:DSPE:DSPE-PEG2000 (85:5:10, w/w/w) with perfluorobutane gas core; encapsulated CPP–siRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HT-1080 human fibrosarcoma cells |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro functional RNA effect (qRT-PCR c-myc mRNA suppression) |
| Output Value | Gene silencing observed; +US enhanced vs -US |
| Output Units | |
| Output Notes | US pre-treatment of NBs before cell exposure (1 MHz, 1 W/cm^2, 10 s on/10 s off, total 60 s). |
| Toxicity Notes | |
| Curation Notes |