Sequence: CKRRMKWKK
| Experiment Id | EXP001180 |
|---|---|
| Paper | Co-delivery of CPP decorated doxorubicin and CPP decorated siRNA by NGR-modified nanobubbles for imp |
| Peptide | CPP |
| Delivery Success Class | no |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Encapsulated CPP–siRNA |
| Rna Concentration | 100 nM siRNA; 4 h treatment then 48 h before qRT-PCR |
| Mixing Ratio | |
| Formulation Format | NGR-modified nanobubbles containing CPP–siRNA (CPP–siRNA/NGR-NB) |
| Formulation Components | NBs (HSPC:DSPE:DSPE-PEG2000-NGR 85:5:10, w/w/w) decorated with NGR (CYGGRGNG); encapsulated CPP–siRNA; perfluorobutane gas core |
| Size Nm | 245.20 |
| Zeta Mv | 2.84 |
| Model Scope | in_vivo |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HT-1080 human fibrosarcoma cells |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro functional RNA effect (qRT-PCR c-myc mRNA suppression) |
| Output Value | Enhanced c-myc suppression; strongest reported for CPP–siRNA/NGR-NB(+US) |
| Output Units | |
| Output Notes | NGR decoration improved activity; CPP–siRNA/NGR-NB(+US) gave the strongest c-myc suppression. |
| Toxicity Notes | |
| Curation Notes |