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EXP001182

Paper

Co-delivery of CPP decorated doxorubicin and CPP decorated siRNA by NGR-modified nanobubbles for improving anticancer therapy (2021)

Peptide

CPP

Sequence: CKRRMKWKK

RNA

siRNA

All experiment fields

Experiment Id EXP001182
Paper Co-delivery of CPP decorated doxorubicin and CPP decorated siRNA by NGR-modified nanobubbles for imp
Peptide CPP
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence
Peptide Concentration Encapsulated CPP–siRNA
Rna Concentration 1.2 mg/kg siRNA (in vivo dose)
Mixing Ratio
Formulation Format CPP–siRNA/NGR-NB with ultrasound (+US)
Formulation Components NGR-modified NBs containing CPP–siRNA; NGR (CYGGRGNG) targeting; US-triggered release/sonoporation
Size Nm 245.20
Zeta Mv 2.84
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model Male nude mice bearing subcutaneous HT-1080 tumors (~200 mm^3 pre-treatment)
Administration Route Tail vein injection every 3 days; +US groups: tumor sonication 30 min post-injection (1 MHz, 1 W/cm^2, 10 s on/10 s off, total 60 s)
Output Type In vivo therapeutic effect (tumor growth inhibition)
Output Value Significant tumor growth inhibition vs control
Output Units
Output Notes CPP–siRNA/NGR-NB(+US) inhibited tumor growth in HT-1080 xenograft model.
Toxicity Notes
Curation Notes