EXP001189

Paper

Design, Synthesis, and Evaluation of Oleyl-WRH Peptides for siRNA Delivery (2024)

Peptide

Sequence: WRHWRH

RNA

All experiment fields

Experiment Id	EXP001189
Paper	Design, Synthesis, and Evaluation of Oleyl-WRH Peptides for siRNA Delivery
Peptide	(WRH)2
Delivery Success Class	no
In Vivo Flag	no
Uptake Confirmed	yes
Label Confidence	high
In Vitro Functional Effect
Endosomal Escape Evidence
Peptide Concentration	Variable to achieve N/P ratios (typically N/P 40 used for uptake/confocal comparisons)
Rna Concentration	Alexa Fluor 488-labeled scrambled siRNA; complexes incubated 24 h (flow cytometry/confocal).; Final siRNA concentration 50 nM for STAT3 silencing (48 h) in MDA-MB-231 and SK-OV-3. (silencing reported mainly for oleyl-(WRH)3/4)
Mixing Ratio	N/P ratio used primarily 40 for uptake/confocal/silencing; complexes formed by mixing peptide (cationic) with siRNA in HBSS, 30 min at room temperature.
Formulation Format	Electrostatic peptide/siRNA nanocomplex
Formulation Components	(WRH)2 + siRNA (complexed via ionic interactions; HBSS; 30 min RT)
Size Nm	71.43
Zeta Mv	13.00
Model Scope	in_vitro
Model Type	in vitro
Cell Lines Or Primary Cells	MDA-MB-231, MCF-7, SK-OV-3, HEK-293 (cytotoxicity); MDA-MB-231 and SK-OV-3 (uptake/flow cytometry; confocal); MDA-MB-231 and SK-OV-3 (STAT3 Western blot).
Animal Model
Administration Route
Output Type	Uptake/internalization (flow cytometry/confocal) and nanoparticle characterization; no STAT3 knockdown data for this peptide reported
Output Value	siRNA uptake observed (flow cytometry/confocal); functional STAT3 silencing not reported for non-oleyl peptides in Western blot section
Output Units
Output Notes	Non-oleyl peptides formed <79 nm complexes at N/P 40 with positive zeta potentials; uptake increased with higher arginine content and oleylation.
Toxicity Notes	Non-cytotoxic up to N/P 80 across tested cell lines; dose-dependent toxicity at higher N/P ratios.
Curation Notes