Design, Synthesis, and Evaluation of Oleyl-WRH Peptides for siRNA Delivery (2024)
Sequence: WRH
| Experiment Id | EXP001192 |
|---|---|
| Paper | Design, Synthesis, and Evaluation of Oleyl-WRH Peptides for siRNA Delivery |
| Peptide | oleyl-(WRH)1 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | Variable to achieve N/P ratios (typically N/P 40 used for characterization and uptake) |
| Rna Concentration | Alexa Fluor 488-labeled scrambled siRNA; complexes incubated 24 h (flow cytometry/confocal). |
| Mixing Ratio | N/P ratio used primarily 40 for uptake/confocal/silencing; complexes formed by mixing peptide (cationic) with siRNA in HBSS, 30 min at room temperature. |
| Formulation Format | Electrostatic peptide/siRNA nanocomplex |
| Formulation Components | oleyl-(WRH)1 + siRNA (HBSS; 30 min RT) |
| Size Nm | 75.66 |
| Zeta Mv | 13.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MDA-MB-231, MCF-7, SK-OV-3, HEK-293 (cytotoxicity); MDA-MB-231 and SK-OV-3 (uptake/flow cytometry; confocal); MDA-MB-231 and SK-OV-3 (STAT3 Western blot). |
| Animal Model | |
| Administration Route | |
| Output Type | Uptake/internalization (flow cytometry) and nanoparticle characterization; STAT3 knockdown not tested |
| Output Value | Uptake observed (flow cytometry with Alexa488-siRNA); oleylation increased uptake vs non-oleyl analogs |
| Output Units | |
| Output Notes | Oleyl chain enhanced interactions with membranes; peptides show serum stability and siRNA binding at N/P ≥ 40. |
| Toxicity Notes | Non-cytotoxic up to N/P 80 across tested cell lines; dose-dependent toxicity at higher N/P ratios. |
| Curation Notes |