Design, Synthesis, and Evaluation of Oleyl-WRH Peptides for siRNA Delivery (2024)
Sequence: WRHWRHWRHWRH
| Experiment Id | EXP001196 |
|---|---|
| Paper | Design, Synthesis, and Evaluation of Oleyl-WRH Peptides for siRNA Delivery |
| Peptide | oleyl-(WRH)4 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | N/P 40 (selected as safer ratio for assays) |
| Rna Concentration | Alexa Fluor 488-labeled scrambled siRNA; complexes incubated 24 h (flow cytometry/confocal).; Final siRNA concentration 50 nM for STAT3 silencing (48 h) in MDA-MB-231 and SK-OV-3. |
| Mixing Ratio | N/P ratio used primarily 40 for uptake/confocal/silencing; complexes formed by mixing peptide (cationic) with siRNA in HBSS, 30 min at room temperature. |
| Formulation Format | Electrostatic peptide/siRNA nanocomplex |
| Formulation Components | oleyl-(WRH)4 + siRNA (HBSS; 30 min RT) |
| Size Nm | 58.74 |
| Zeta Mv | 13.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MDA-MB-231 |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro functional RNA effect (STAT3 protein by Western blot) + uptake (flow cytometry/confocal) |
| Output Value | Uptake: ~75% fluorescent-positive cells (Alexa488-siRNA) at N/P 40 after 24 h in MDA-MB-231 and SK-OV-3; STAT3 silencing: ~75% STAT3 downregulation (Western blot) at N/P 40 |
| Output Units | |
| Output Notes | Confocal microscopy described cytosolic delivery; oleyl-(WRH)4 showed the highest uptake and STAT3 knockdown among tested peptides. |
| Toxicity Notes | Minimal cytotoxicity at N/P ≤ 80; selected N/P 40 for functional assays. |
| Curation Notes |