Delivery and tracking of quantum dot peptide bioconjugates in an intact developing avian brain (2015)
JB577 (QD-PEG-JB577 peptide–QD bioconjugate (DHLA-PEG coat; His6-ZnS coordination))
Sequence: WGDap(Palmitoyl)VKIKKP9GGH6
| Experiment Id | EXP001238 |
|---|---|
| Paper | Delivery and tracking of quantum dot peptide bioconjugates in an intact developing avian brain |
| Peptide | JB577 (QD-PEG-JB577 peptide–QD bioconjugate (DHLA-PEG coat; His6-ZnS coordination)) |
| Delivery Success Class | no |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | ~1.25–2.5 µM (25 peptides/QD; QD 50–100 nM) |
| Rna Concentration | |
| Mixing Ratio | 25:1 peptide:QD |
| Formulation Format | QD-PEG-JB577 peptide–QD bioconjugate (DHLA-PEG coat; His6-ZnS coordination) |
| Formulation Components | CdSe/ZnS core-shell QD (625 nm emission) with DHLA-PEG ligand; JB577 palmitoylated lipopeptide attached via His6; prepared in 5% DMSO/PBS then diluted into complete growth medium |
| Size Nm | 6.00 |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Chicken (Gallus gallus) embryos; microinjection at embryonic day 4 (E4; stage 21) |
| Administration Route | In ovo microinjection into embryonic spinal cord canal (E4) |
| Output Type | uptake / biodistribution |
| Output Value | E6 spinal cord sections after E4 injection: QD signal present but less uniformly dispersed; PEG-coated QDs show more clustering vs CL4. |
| Output Units | |
| Output Notes | Figure 2 shows more uniform dispersion for CL4 vs PEG; clusters appeared more frequently with PEG-coated QDs. |
| Toxicity Notes | No difference in mortality vs dye-only controls; no gross physical differences; normal expression patterns of neuronal/glial markers; chicks hatched on schedule with normal behavior. |
| Curation Notes |