Hph1 + cl-KALA (self-crosslinked fusogenic KALA)
Sequence: Hph1:YARVRRRGPRR | cl-KALA monomer:CWEAKLAKALAKALAKHLAKALAKALKAC
| Experiment Id | EXP001240 |
|---|---|
| Paper | Multifunctional siRNA Delivery System: Polyelectrolyte Complex Micelles of Six-arm PEG Conjugate of |
| Peptide | Hph1 + cl-KALA (self-crosslinked fusogenic KALA) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 75 pmol (per well, transfection assay) |
| Mixing Ratio | 16 |
| Formulation Format | polyelectrolyte complex micelles (PEG-siRNA conjugate complexed with peptide) |
| Formulation Components | 6-arm PEG-disulfide-siRNA conjugate (6PEG-siRNA) functionalized with Hph1 CPP (6PEG-siRNA-Hph1) electrostatically complexed with cl-KALA |
| Size Nm | 178.45 |
| Zeta Mv | 21.50 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MDA-MB-435-GFP (human breast cancer cell line stably expressing GFP) |
| Animal Model | |
| Administration Route | |
| Output Type | GFP expression (% of untreated control; lower = more silencing) |
| Output Value | 31.3 ± 5.6% GFP expression at N/P 16 (10% serum) |
| Output Units | |
| Output Notes | Transfection 4 h in 10% fresh mouse serum DMEM; readout at 36 h post-transfection via GFP fluorescence; DLS size 178.45±32.55 nm, zeta +21.5±1.0 mV (N/P 16) |
| Toxicity Notes | Reported reduced/no cytotoxicity for cl-KALA-based complexes (compared with KALA) |
| Curation Notes |