cl-KALA (self-crosslinked fusogenic KALA)
Sequence: cl-KALA monomer:CWEAKLAKALAKALAKHLAKALAKALKAC
| Experiment Id | EXP001242 |
|---|---|
| Paper | Multifunctional siRNA Delivery System: Polyelectrolyte Complex Micelles of Six-arm PEG Conjugate of |
| Peptide | cl-KALA (self-crosslinked fusogenic KALA) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 75 pmol (per well, transfection assay) |
| Mixing Ratio | 16 |
| Formulation Format | siRNA/peptide electrostatic complex (polyplex) |
| Formulation Components | Naked GFP siRNA electrostatically complexed with cl-KALA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MDA-MB-435-GFP (human breast cancer cell line stably expressing GFP) |
| Animal Model | |
| Administration Route | |
| Output Type | GFP expression (% of untreated control; lower = more silencing) |
| Output Value | 81.4 ± 1.5% GFP expression at N/P 16 (10% serum) |
| Output Units | |
| Output Notes | Transfection 4 h in 10% fresh mouse serum DMEM; readout at 36 h post-transfection via GFP fluorescence |
| Toxicity Notes | Not specifically highlighted; main comparison shows lower efficacy than PEG-conjugate systems |
| Curation Notes |