Surfactant Protein B Promotes Cytosolic SiRNA Delivery by Adopting a Virus-like Mechanism of Action (2021)
Sequence: KLLLLKLLLLKLLLLKLLLLK
| Experiment Id | EXP001250 |
|---|---|
| Paper | Surfactant Protein B Promotes Cytosolic SiRNA Delivery by Adopting a Virus-like Mechanism of Action |
| Peptide | KL4 (sinapultide) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | 0.04 mol% in lipid coat (matched to SP-B mol fraction) |
| Rna Concentration | 100 nM |
| Mixing Ratio | siNGs layered with lipid coat DOPC:PG 85:15 (wt%) supplemented with KL4 (0.04 mol%) |
| Formulation Format | peptide-supplemented lipid-coated nanogel (siNGs-LIP:KL4) |
| Formulation Components | siRNA-loaded dextran nanogel + lipid coat (DOPC:PG 85:15 wt%) + KL4 peptide |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | H1299_eGFP |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | Little/no knockdown; eGFP expression ~80–90% |
| Output Units | |
| Output Notes | KL4 did not replicate SP-B effect on siRNA delivery (Fig. 2b). |
| Toxicity Notes | |
| Curation Notes |