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EXP001254

Paper

The endosomal escape vehicle platform enhances delivery of oligonucleotides in preclinical models of neuromuscular disorders (2023)

Peptide

EEV1

Sequence: Proprietary cyclic CPP (sequence not disclosed)

RNA

PMO (antisense oligonucleotide)

All experiment fields

Experiment Id EXP001254
Paper The endosomal escape vehicle platform enhances delivery of oligonucleotides in preclinical models of
Peptide EEV1
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration 10 mg/kg (PMO equiv) ×3 daily
Mixing Ratio
Formulation Format covalent peptide–PMO conjugate
Formulation Components EEV1-PMO654
Size Nm
Zeta Mv
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model EGFP-654 transgenic mice
Administration Route intramuscular (i.m.)
Output Type splice correction / EGFP fluorescence
Output Value ~82% splice correction near injection site (RT-PCR densitometry)
Output Units
Output Notes Diffuse EGFP fluorescence observed in muscle near injection site (functional reporter restoration).
Toxicity Notes
Curation Notes