Sequence: WWWWWEEEEACA + HHHHHHHH
| Experiment Id | EXP001263 |
|---|---|
| Paper | Development of a multi-level pH-responsive lipid nanoplatform for efficient co-delivery of siRNA and |
| Peptide | Cyc(E4W5ACA) + SA-H8 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | 100 nM |
| Mixing Ratio | SA-H8/siRNA N/P=5:1 |
| Formulation Format | Cyc-LH/si (cyclic peptide–modified liposome; SA-H8/siRNA complex encapsulated) |
| Formulation Components | liposome core: DOPE/CHEMS/Chol; PEGylation: DSPE-PEG2000; DSPE-PEG2000-Cyc(E4W5ACA); SA-H8/siPLK-1 |
| Size Nm | 150.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MCF-7; A549 |
| Animal Model | |
| Administration Route | |
| Output Type | PLK-1 knockdown / apoptosis |
| Output Value | |
| Output Units | |
| Output Notes | qRT-PCR & western blot show PLK-1 suppression (stronger at pH 6.5); induces apoptosis; pH-responsive uptake and lysosomal escape characterized by confocal/flow cytometry |
| Toxicity Notes | |
| Curation Notes |