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EXP001267

Paper

Development of a multi-level pH-responsive lipid nanoplatform for efficient co-delivery of siRNA and small-molecule drugs in tumor treatment (2024)

Peptide

Cyc(E4W5ACA) + SA-H8

Sequence: WWWWWEEEEACA + HHHHHHHH

RNA

siRNA

All experiment fields

Experiment Id EXP001267
Paper Development of a multi-level pH-responsive lipid nanoplatform for efficient co-delivery of siRNA and
Peptide Cyc(E4W5ACA) + SA-H8
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence yes
Peptide Concentration
Rna Concentration 100 nM siRNA; 0.1 µg/mL DTX
Mixing Ratio SA-H8/siRNA N/P=5:1
Formulation Format Cyc-LH/DTX-si (co-delivery liposome)
Formulation Components liposome core: DOPE/CHEMS/Chol; PEGylation: DSPE-PEG2000; DSPE-PEG2000-Cyc(E4W5ACA); SA-H8/siPLK-1; docetaxel
Size Nm 150.00
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells MCF-7
Animal Model
Administration Route
Output Type Apoptosis (co-delivery synergy)
Output Value
Output Units
Output Notes Co-delivery increases apoptosis (~53% reported) vs single-agent controls; demonstrates functional siRNA delivery plus chemo synergy.
Toxicity Notes
Curation Notes