Sequence: WWWWWEEEEACA + HHHHHHHH
| Experiment Id | EXP001269 |
|---|---|
| Paper | Development of a multi-level pH-responsive lipid nanoplatform for efficient co-delivery of siRNA and |
| Peptide | Cyc(E4W5ACA) + SA-H8 |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | 0.28 mg/kg siRNA; 0.86 mg/kg DTX |
| Mixing Ratio | SA-H8/siRNA N/P=5:1 |
| Formulation Format | Cyc-LH/DTX-si (systemic co-delivery) |
| Formulation Components | liposome core: DOPE/CHEMS/Chol; PEGylation: DSPE-PEG2000; DSPE-PEG2000-Cyc(E4W5ACA); SA-H8/siPLK-1; docetaxel |
| Size Nm | 150.00 |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | BALB/c nude mice with MCF-7 xenograft tumors |
| Administration Route | i.v. (tail vein) |
| Output Type | Tumor growth inhibition (co-delivery) |
| Output Value | |
| Output Units | |
| Output Notes | Best tumor suppression among groups; PLK-1 silencing in tumor tissue; increased apoptosis (TUNEL). siRNA 0.28 mg/kg; DTX 0.86 mg/kg; dosing once every other day for 10 days (tumor suppression study) |
| Toxicity Notes | No significant body-weight loss reported |
| Curation Notes |