Sequence: WWWWWEEEEACA + RRRRRRRR
| Experiment Id | EXP001270 |
|---|---|
| Paper | Development of a multi-level pH-responsive lipid nanoplatform for efficient co-delivery of siRNA and |
| Peptide | Cyc(E4W5ACA) + SA-R8 |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | 0.28 mg/kg |
| Mixing Ratio | |
| Formulation Format | Cyc-L/si (systemic) |
| Formulation Components | liposome core: DOPE/CHEMS/Chol; PEGylation: DSPE-PEG2000; DSPE-PEG2000-Cyc(E4W5ACA); SA-R8/siPLK-1 |
| Size Nm | 150.00 |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | BALB/c nude mice with MCF-7 xenograft tumors |
| Administration Route | i.v. (tail vein) |
| Output Type | Tumor growth inhibition / PLK-1 silencing |
| Output Value | |
| Output Units | |
| Output Notes | Inhibits tumor growth vs controls; PLK-1 mRNA/protein reduced in tumors; Cyc modification improves efficacy vs non-Cyc. siRNA 0.28 mg/kg; DTX 0.86 mg/kg; dosing once every other day for 10 days (tumor suppression study) |
| Toxicity Notes | No significant body-weight loss reported |
| Curation Notes |