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EXP001271

Paper

Development of a multi-level pH-responsive lipid nanoplatform for efficient co-delivery of siRNA and small-molecule drugs in tumor treatment (2024)

Peptide

SA-H8

Sequence: HHHHHHHH

RNA

siRNA

All experiment fields

Experiment Id EXP001271
Paper Development of a multi-level pH-responsive lipid nanoplatform for efficient co-delivery of siRNA and
Peptide SA-H8
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed no
Label Confidence medium
In Vitro Functional Effect
Endosomal Escape Evidence yes
Peptide Concentration
Rna Concentration 0.28 mg/kg
Mixing Ratio SA-H8/siRNA N/P=5:1
Formulation Format N-LH/si (systemic)
Formulation Components liposome core: DOPE/CHEMS/Chol; PEGylation: DSPE-PEG2000; SA-H8/siPLK-1
Size Nm 170.00
Zeta Mv
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model BALB/c nude mice with MCF-7 xenograft tumors
Administration Route i.v. (tail vein)
Output Type Tumor growth inhibition / PLK-1 silencing
Output Value
Output Units
Output Notes Liposome group with siPLK-1 reduces PLK-1 expression in tumor and inhibits growth; less effective than Cyc-modified formulations. siRNA 0.28 mg/kg; DTX 0.86 mg/kg; dosing once every other day for 10 days (tumor suppression study)
Toxicity Notes No significant body-weight loss reported
Curation Notes