A gene delivery system for insect cells mediated by arginine-rich cell-penetrating peptides (2012)
Sequence: C-5H-R9-5H-C
| Experiment Id | EXP001284 |
|---|---|
| Paper | A gene delivery system for insect cells mediated by arginine-rich cell-penetrating peptides |
| Peptide | HR9 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | N/P=3 (amount set by N/P ratio; see Methods) |
| Rna Concentration | 3 µg plasmid DNA per well (24-well plate); N/P=3 |
| Mixing Ratio | N/P=3 (NH3+/PO4−) |
| Formulation Format | noncovalent CPP/pDNA complexes |
| Formulation Components | CPP (SR9/HR9/PR9) + plasmid DNA in PBS; 2 h incubation |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Sf9 (Spodoptera frugiperda) insect cells |
| Animal Model | |
| Administration Route | |
| Output Type | reporter expression (EGFP) / transfection efficiency |
| Output Value | 18.76 ± 1.09% GFP+ cells (flow cytometry; N/P=3) |
| Output Units | |
| Output Notes | EGFP/RFP expression observed by fluorescence microscopy; EGFP transcripts confirmed by RT-PCR. Cy3-labeled DNA delivery visualized by confocal microscopy. |
| Toxicity Notes | CPPs and CPP/DNA complexes showed 84–93% cell viability (MTT assay; 3 days). |
| Curation Notes |