Sequence: RRRRRRRRR
| Experiment Id | EXP001287 |
|---|---|
| Paper | Attachment of Cell-Binding Ligands to Arginine-Rich Cell-Penetrating Peptides Enables Cytosolic Tran |
| Peptide | 9LR |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | no |
| Peptide Concentration | 1–5 µM (ratios 5–25; typically 10:1 used) |
| Rna Concentration | ~200 nM (100 pmol in 0.5 mL for Neuro2a); Jurkat 100 pmol in 0.2 mL (~500 nM) |
| Mixing Ratio | 10:1 (peptide:siRNA molar) |
| Formulation Format | electrostatic peptide:siRNA complex |
| Formulation Components | 9LR + siRNA (noncovalent complex) |
| Size Nm | 227.00 |
| Zeta Mv | -37.30 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Neuro2a (mouse neuroblastoma; GFP-expressing) |
| Animal Model | |
| Administration Route | in vitro (cell incubation) |
| Output Type | gene knockdown (GFP reporter) |
| Output Value | No significant GFP silencing at 10:1; required ≥20:1 peptide:siRNA |
| Output Units | |
| Output Notes | Uptake low at 10:1 (MFI 11.2 ± 1.4 for 9LR:siRNA). Confocal shows predominantly endosomal/vesicular localization (supp Fig S4B). |
| Toxicity Notes | Not reported for 9LR alone. |
| Curation Notes |