Sequence: scFvCD7 +RRRRRRRRR
| Experiment Id | EXP001290 |
|---|---|
| Paper | Attachment of Cell-Binding Ligands to Arginine-Rich Cell-Penetrating Peptides Enables Cytosolic Tran |
| Peptide | scFvCD7-9DR |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 5 µM (10:1 ratio in Jurkat) |
| Rna Concentration | ~500 nM (100 pmol in 0.2 mL for Jurkat) |
| Mixing Ratio | 10:1 (peptide:siRNA molar) |
| Formulation Format | electrostatic peptide:siRNA complex |
| Formulation Components | scFvCD7-9DR + siRNA (noncovalent complex) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Jurkat (human T cell line) |
| Animal Model | |
| Administration Route | in vitro (cell incubation) |
| Output Type | gene knockdown (CD4 mRNA, qPCR) |
| Output Value | 64.5 ± 5.9% CD4 mRNA reduction at 4 h (vs siLuc control) |
| Output Units | |
| Output Notes | Enhanced uptake into Jurkat (MFI 70.83 ± 26.05). Native 9DR and Lipofectamine 2000 failed to knock down CD4 in Jurkat. |
| Toxicity Notes | Not reported. |
| Curation Notes |