Sequence: RRRRRRRR
| Experiment Id | EXP001292 |
|---|---|
| Paper | The effect of dexamethasone/cell-penetrating peptide nanoparticles on gene delivery for inner ear th |
| Peptide | Arg8 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | medium |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | 0.04 |
| Formulation Format | polymeric micelle polyplex (nanoparticle) |
| Formulation Components | PHEA-g-C18-Arg8 (PCA) polymeric micelles (C18 hydrophobic core; Arg8 shell) electrostatically complexing nucleic acids |
| Size Nm | 138.00 |
| Zeta Mv | 25.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HEI-OC1 (immortalized mouse organ of Corti cells) |
| Animal Model | |
| Administration Route | |
| Output Type | reporter expression |
| Output Value | Higher GFP expression than Lipofectamine; best at w/w 0.04 (~1.5× vs Lipofectamine) |
| Output Units | |
| Output Notes | FACS measured GFP fluorescence at 24 h; DNA:micelle weight ratios tested 0.04/0.08/0.1 and 0.04 selected for subsequent experiments. |
| Toxicity Notes | |
| Curation Notes |