Sequence: RRRRRRRR
| Experiment Id | EXP001293 |
|---|---|
| Paper | The effect of dexamethasone/cell-penetrating peptide nanoparticles on gene delivery for inner ear th |
| Peptide | Arg8 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | medium |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | 0.04 |
| Formulation Format | Dex-loaded polymeric micelle polyplex (nanoparticle) |
| Formulation Components | PCA micelles with dexamethasone (Dex) loaded in hydrophobic core; nucleic acids complexed on surface via Arg8 |
| Size Nm | 130.00 |
| Zeta Mv | 22.50 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HEI-OC1 (immortalized mouse organ of Corti cells) |
| Animal Model | |
| Administration Route | |
| Output Type | reporter expression |
| Output Value | GFP expression mildly increased vs Dex-free PCA/GFP; both > Lipofectamine |
| Output Units | |
| Output Notes | PCA/Dex/GFP at w/w 0.04 compared to PCA/GFP and Lipofectamine; GFP quantified by mean fluorescence intensity (FACS). |
| Toxicity Notes | |
| Curation Notes |