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EXP001298

Paper

Photochemically enhanced delivery of a cell-penetrating peptide nucleic acid conjugate targeting human telomerase reverse transcriptase: effects on telomere status and proliferative potential of human prostate cancer cells (2007)

Peptide

Tat

Sequence: RKKRRQRRR

RNA

PNA (antisense)

All experiment fields

Experiment Id EXP001298
Paper Photochemically enhanced delivery of a cell-penetrating peptide nucleic acid conjugate targeting hum
Peptide Tat
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed no
Label Confidence medium
In Vitro Functional Effect no
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration
Mixing Ratio
Formulation Format PNA–CPP conjugate + photochemical internalization (PCI)
Formulation Components hTERT-PNA-Tat + photosensitizer TPPS2a (0.7 µg/mL, 18 h) then 4 h chase, then blue light 90 s (≈405 nm peak) to rupture endosomal membranes
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells U2-OS (hTERT-negative osteosarcoma)
Animal Model
Administration Route
Output Type negative control
Output Value No specific growth inhibition attributable to hTERT targeting (hTERT-negative line)
Output Units
Output Notes Used 2 µM hTERT-PNA-Tat in PCI schedule; growth inhibition similar to photochemical treatment alone.
Toxicity Notes
Curation Notes