Sequence: PKPKPKPKPKPKPKPKPKPKPKPKPKPKPKPKPKPKPKPK
| Experiment Id | EXP001302 |
|---|---|
| Paper | Widespread displacement of DNA- and RNA-binding factors underlies toxicity of arginine-rich cell-pen |
| Peptide | (PK)20 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | 7.5 µM |
| Rna Concentration | 2 µM |
| Mixing Ratio | 7.5 µM peptide : 2 µM RNA (molar ~3.75:1) |
| Formulation Format | simple co-incubation (non-covalent complex) |
| Formulation Components | |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | U2OS (human osteosarcoma) cells |
| Animal Model | |
| Administration Route | |
| Output Type | uptake (nuclear Cy5-ssRNA levels) |
| Output Value | Nuclear Cy5-ssRNA signal quantified (HTM) after peptide co-treatment |
| Output Units | |
| Output Notes | High-throughput microscopy quantification of nuclear Cy5-ssRNA after 8 h co-treatment with CPPs (Appendix Figure S3F). |
| Toxicity Notes | Arginine-rich (PR)20 and (GR)20 show cellular toxicity in clonogenic/viability assays; controls (GA)20 and (PK)20 less toxic (Appendix Figure S3G; S4). |
| Curation Notes |