A novel cell-penetrating peptide to facilitate intercellular transport of fused proteins (2014)
Sequence: YARAAARQARAYARAAARQARAYARAAARQARA
| Experiment Id | EXP001311 |
|---|---|
| Paper | A novel cell-penetrating peptide to facilitate intercellular transport of fused proteins |
| Peptide | Tatm3x |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | expressed secretory CPP-fusion protein (signal peptide) / intercellular transport |
| Formulation Components | SP-Tatm3xCherry produced in H1299; lysate/supernatant applied to A549; intercellular transport tested via mixing and membrane-separated co-culture; secretion blocked by Brefeldin A. |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | A549 (receiving); fusion protein produced in H1299 producer cells |
| Animal Model | |
| Administration Route | |
| Output Type | protein transduction / intercellular transport |
| Output Value | Robust uptake (red fluorescence) and intercellular transport of mCherry fusion protein. |
| Output Units | |
| Output Notes | Lysate-derived SP-Tatm3xCherry shows strong transduction; supernatant-derived shows lower but detectable transduction. |
| Toxicity Notes | |
| Curation Notes |