A novel cell-penetrating peptide to facilitate intercellular transport of fused proteins (2014)
Sequence: YGRKKRRQRRR
| Experiment Id | EXP001312 |
|---|---|
| Paper | A novel cell-penetrating peptide to facilitate intercellular transport of fused proteins |
| Peptide | Tat (basic domain) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | expressed secretory CPP-fusion protein (signal peptide) / intercellular transport |
| Formulation Components | SP-TatCherry expressed in H1299; lysate applied to A549 (supernatant not used due to minimal secretion). |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | A549 (receiving); fusion protein produced in H1299 producer cells |
| Animal Model | |
| Administration Route | |
| Output Type | protein transduction |
| Output Value | Uptake (red fluorescence) observed in A549 cells after treatment with lysate-derived SP-TatCherry. |
| Output Units | |
| Output Notes | Tat transduction inhibited by cellular lysate/RNA; RNase restores activity (RNA inhibition). |
| Toxicity Notes | |
| Curation Notes |