A novel cell-penetrating peptide to facilitate intercellular transport of fused proteins (2014)
Sequence: YARAAARQARAYARAAARQARAYARAAARQARA
| Experiment Id | EXP001314 |
|---|---|
| Paper | A novel cell-penetrating peptide to facilitate intercellular transport of fused proteins |
| Peptide | Tatm3x |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | expressed secretory CPP-fusion protein (signal peptide) / intercellular transport |
| Formulation Components | SP-Tatm3xBH3Bak expressed in H1299 producer cells; cleared lysate applied to fresh H1299; spatial co-culture (IdMOC) used to test bystander effect. |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | H1299 (receiving cells in viability assay / IdMOC co-culture) |
| Animal Model | |
| Administration Route | |
| Output Type | functional protein effect |
| Output Value | Reduced cell viability in receiving cells (WST-1); bystander reduction in co-culture (~79% vs control). |
| Output Units | |
| Output Notes | Demonstrates Tatm3x-mediated entry and function of fused pro-apoptotic peptide; control SP-Tatm3xV5 minimal effect. |
| Toxicity Notes | |
| Curation Notes |