Amphipathic Octenyl-Alanine Modified Peptides Mediate Effective siRNA Delivery (2025)
Sequence: H-L{R8}KLAKA{R8}AKLLKA{S8}AKAL-NH2
| Experiment Id | EXP001321 |
|---|---|
| Paper | Amphipathic Octenyl-Alanine Modified Peptides Mediate Effective siRNA Delivery |
| Peptide | hPep3 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | MR30 (N/P 4.3) |
| Formulation Format | peptide/siRNA nanoparticles (electrostatic complexes) |
| Formulation Components | peptide + siRNA in HBG (HEPES-buffered glucose) / cell culture media; complexes at MR30 |
| Size Nm | 80.00 |
| Zeta Mv | 18.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | RAW 264.7 murine macrophages |
| Animal Model | |
| Administration Route | |
| Output Type | protein knockdown (flow cytometry) |
| Output Value | >50% CD45 protein downregulation at 100 nM siCD45 (24 h, MR30) |
| Output Units | |
| Output Notes | CD45 expression measured by flow cytometry after 24 h treatment with hPep3/siCD45 NPs; siCTRL used as negative control. |
| Toxicity Notes | No specific toxicity reported for RAW264.7 in CD45 assay; overall WST-1 in HEK-Luc showed minimal cytotoxicity up to 200 nM. |
| Curation Notes |