Gene delivery using cell penetrating peptides-zeolitic imidazolate frameworks (2020)
Sequence: Stearyl-AGYLLGKLL OOLAAAALOOLL-NH2
| Experiment Id | EXP001343 |
|---|---|
| Paper | Gene delivery using cell penetrating peptides-zeolitic imidazolate frameworks |
| Peptide | PF14 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | medium |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | pGL3 205 ng/µL (4 µL used in complex prep) |
| Mixing Ratio | Stepwise mixing with ZIF-8 (4 µL of 1 mg/mL) + pGL3 (4 µL); plasmid:ZIF-8 tested 1:0.5 and 1:1; charge ratios 5 and 10 (reported across experiments) |
| Formulation Format | CPP/ZIF-8/RNA polyplex (noncovalent) |
| Formulation Components | PF14 + pGL3 |
| Size Nm | 25.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa |
| Animal Model | |
| Administration Route | |
| Output Type | Reporter gene expression (luciferase) |
| Output Value | |
| Output Units | |
| Output Notes | Baseline transfection with peptide alone used for normalization; ZIF-8 variants compared against this control. |
| Toxicity Notes | WST-1 assay in HeLa cells showed no observable toxicity for ZIF-8 composites with or without CPPs under tested conditions (24 h incubation). |
| Curation Notes |