Gene delivery using cell penetrating peptides-zeolitic imidazolate frameworks (2020)
Sequence: Stearyl-AGYLLGKLL OOLAAAALOOLL-NH2
splice-correction oligonucleotide (SCO/ASO)
| Experiment Id | EXP001356 |
|---|---|
| Paper | Gene delivery using cell penetrating peptides-zeolitic imidazolate frameworks |
| Peptide | PF14 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | medium |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 100 nM SCO (MR10) used for confocal imaging; transfection assays used SCO in similar reporter setup (concentration not always specified per figure) |
| Mixing Ratio | Stepwise complexation with/without ZIF-8 nanocomposite; SCO:ZIF-8 ratios tested 1:0.5 and 1:1 (supplementary figures) |
| Formulation Format | CPP/ZIF-8/ASO polyplex (noncovalent) |
| Formulation Components | PF14 + SCO + ZIF-8 |
| Size Nm | 25.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa pLuc705 (HeLa 705) |
| Animal Model | |
| Administration Route | |
| Output Type | Splice correction (luciferase readout) |
| Output Value | |
| Output Units | |
| Output Notes | Ternary complexes containing ZIF-8 nanocomposites showed higher splice-correction activity than CPP+SCO alone; PF14+ZIF-8 reported to outperform Lipofectamine 2000 in this assay. |
| Toxicity Notes | WST-1 assay in HeLa cells showed no observable toxicity for ZIF-8 composites with or without CPPs under tested conditions (24 h incubation). |
| Curation Notes |