Gene delivery using cell penetrating peptides-zeolitic imidazolate frameworks (2020)
Sequence: Stearyl-AGYLLGKLL OOLAAAALOOLL-NH2
| Experiment Id | EXP001370 |
|---|---|
| Paper | Gene delivery using cell penetrating peptides-zeolitic imidazolate frameworks |
| Peptide | PF14 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | medium |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 10 and 25 nM siRNA tested |
| Mixing Ratio | siRNA:PF14 molar ratio 1:20; with and without ZIF-8 composites |
| Formulation Format | CPP/ZIF-8/siRNA polyplex (noncovalent) |
| Formulation Components | PF14 + siRNA + BSA@ZIF-8 |
| Size Nm | 25.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | U-87 MG-luc2 |
| Animal Model | |
| Administration Route | |
| Output Type | Gene knockdown (luciferase reduction) |
| Output Value | |
| Output Units | |
| Output Notes | Significant knockdown reported using CPPs-ZIF-8 vectors (10–25 nM siRNA); ZIF-8 variants compared vs PF14-siRNA alone. |
| Toxicity Notes | WST-1 assay in HeLa cells showed no observable toxicity for ZIF-8 composites with or without CPPs under tested conditions (24 h incubation). |
| Curation Notes |