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EXP001373

Paper

Chitosan enhances gene delivery of oligonucleotide complexes with magnetic nanoparticles–cell-penetrating peptide (2018)

Peptide

PF14

Sequence: AGYLLGKLLOOLAAAALOOLL

RNA

plasmid DNA

All experiment fields

Experiment Id EXP001373
Paper Chitosan enhances gene delivery of oligonucleotide complexes with magnetic nanoparticles–cell-penetr
Peptide PF14
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration pGL3 (amount not clearly stated for transfection)
Mixing Ratio CR 5:1 (CPP:pGL3)
Formulation Format noncovalent CPP–RNA complex
Formulation Components
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells HeLa
Animal Model
Administration Route
Output Type luciferase reporter expression
Output Value relative luciferase (normalized)
Output Units
Output Notes PF14–pGL3 transfection measured by luciferase assay; PF14–CTS@MNPs improved transfection vs PF14 alone (relative data).
Toxicity Notes WST-1 assay: complexes reported biocompatible / no observed cytotoxicity in HeLa cells.
Curation Notes