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EXP001377

Paper

Chitosan enhances gene delivery of oligonucleotide complexes with magnetic nanoparticles–cell-penetrating peptide (2018)

Peptide

PF14

Sequence: AGYLLGKLLOOLAAAALOOLL

RNA

antisense oligonucleotide

All experiment fields

Experiment Id EXP001377
Paper Chitosan enhances gene delivery of oligonucleotide complexes with magnetic nanoparticles–cell-penetr
Peptide PF14
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration SCO (10 µM used in complex preparation)
Mixing Ratio MR 10:1 (CPP:SCO) (paper also discusses MR20 in results figure)
Formulation Format noncovalent CPP–RNA complex
Formulation Components
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells HeLa puLuc 705
Animal Model
Administration Route
Output Type splice-correction luciferase assay
Output Value relative luminescence (normalized)
Output Units
Output Notes PF14–SCO produced splice-correction activity in vitro; serves as baseline for CTS@MNP enhancement.
Toxicity Notes WST-1 assay: complexes reported biocompatible / no observed cytotoxicity in HeLa cells.
Curation Notes